… location of thioredoxin facilitate the rapid purification for fusion proteins. This expres—sion …
inclusion bodies 2 The solubility—improved target protein is in its wild type non-fusion form, …

… restriction sites for target gene insertion. Fusion proteins can be expressed with E. coli and
… In order to possibly overcome the problem of inclusion body, we will introduce the OsmY (a …

… Researchers then change their research targets to develop AMPs which … fusion proteins
were expressed as inclusion bodies in E. coli. 90% AMPs can be cleaved off from fusion protein …

… to refold the inclusion bodies successfully. Therefore, some … Our results showed that the
hEDN fusion protein with a GST … Furthermore, the target band was further confirmed as hEDN by …

… in inactive protein (ie, inclusion body) and hinder the protein … protein (INP) along with Intein
(INT) and target protein, here … was able to produce fusion protein on cell membrane surface.…

… -Ulp1p-His6,we purified the fusion protein GST-Ulp1p-His6by either … h; 3, 5, 7, 9, 11: inclusion
body induced by 0.25 mmol/L IPTG at 37C … SDS-PAGE analysis of the cleavage of different …

… , we selected a pair of specific primers with cleavage site by C3 gene segment. After ligation
… The target protein was mainly in the form of inclusion bodies. The recombinant protein can …

… targeting moieties suitable for use in cytotoxic fusion proteins… )) were transformed with each
expression plasmid to produce … fusion proteins accumulated in inclusion bodies. These were …

… from the E2 enzymes to target proteins. It was also shown that … We expressed a fusion protein
Pgk1-EGFP in WT cells as an … be released after the cleavage of fusion protein by vacuolar …

… the target gene was expressed at high-level in the form of fusion … The molecular weight of
the fusion protein was 23kDa by … of the purification and cleavage of CD 1, The purified Trx-CD; …